Optimization of Fermentation Conditions for Cellulases Production by Bacillus licheniformis MVS1 and Bacillus sp. MVS3 Isolated from Indian Hot Spring
نویسندگان
چکیده
The aim of this work was to study the effect of some nutritional and environmental factors on the production of cellulases, in particular endoglucanase (CMCase) and exoglucanases (FPase) from Bacillus licheniformis MVS1 and Bacillus sp. MVS3 isolated from an Indian hot spring. The characterization study indicated that the optimum pH and temperature value was 6.5 to 7.0 and 50-55C, respectively. Maximum cellulases production by both the isolates was detected after 60 h incubation period using wheat and rice straw. The combination of inorganic and organic nitrogen source was suitable for cellulases production. Overall, FPase production was much higher than CMCase production by both of the strains. Between the two thermophiles, the cellulolytic activity was more in B. licheniformis MVS1 than Bacillus sp. MVS3 in varying environmental and nutritional conditions.
منابع مشابه
Effect of nutritional and environmental factors on cellulases activity by thermophilic bacteria isolated from hot spring
This study presents effects of some nutritional and environmental factors on cellulases production by isolates, Bacillus licheniformis WBS1 and Bacillus sp. WBS3, which have been isolated from an Indian hot spring. Activity of both CMCase and FPase were higher in case of wheat straw. Maximum enzymatic activity was found at following optimum parameters: pH (8.0 when grown on rice, 9.0 when grown...
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